Biological microbial agents : Inhibit root knot nematode

Scientific Overview

This high-efficacy composite microbial inoculant leverages synergistic strains of Purpureocillium lilacinum and Bacillus subtilis to target Root Knot Nematodes (Meloidogyne spp.), soil-borne pathogens, and soil degradation. Engineered for sustainable intensification, it enhances crop resilience, yield quality, and soil health across diverse agroecosystems while ensuring zero chemical residues.

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NO.1: Technological Superiority & Mechanism of Action

1. Dual-Strain Synergy (Inhibit Root Knot Nematode):

   • Purpureocillium lilacinum: Parasitizes nematode eggs/juveniles via chitinase/protease enzymatic degradation.

   • Bacillus subtilis: Secretes cyclic lipopeptides (surfactin, iturin) suppressing nematode motility and pathogenic fungi (Fusarium, Rhizoctonia).

2. Ultra-High Purity & Viability:
   Precision-controlled submerged fermentation (50L bioreactors, strain-specific pH/temperature protocols) followed by centrifugal concentration (10,000 × g) achieves pre-formulation titers >100 billion CFU/mL. Aseptic processing eliminates contaminant microbes.

3. Soil Function Restoration:

   • Nutrient Solubilization: Organic acid production liberates fixed P/K.

   • Biostimulation: IAA & GA₃ phytohormones boost root biomass by 20–40%.

   • Detoxification: Degrades allelopathic compounds causing continuous cropping障碍 (replant disease).

4. Enhanced Biocontrol Spectrum:
   Induces Systemic Resistance (ISR) via upregulation of pathogenesis-related (PR) proteins and biofilm formation to exclude pathogens.

5. Stability Optimization:
   Chitosan-based microencapsulation (2% w/w) during fluidized-bed drying (45°C) ensures >90% spore germination after 12-month storage.

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NO.2: Precision Application Protocol

Critical Pre-Activation:
Dissolve inoculant in lukewarm water with 1–2% non-sulfured brown sugar. Activate 30–60 min in shade. Note: Sugar metabolism increases microbial ATP synthesis, elevating colonization kinetics 3–5 fold.

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NO.3: Universal Crop Compatibility

*Optimal results verified across 150+ species:*

• Vegetables: Tomato, bell pepper, eggplant, cabbage, broccoli, carrot, potato, cucumber, melon, soybean, snap bean

• Fruits: Citrus, grape, apple, strawberry, banana, mango, blueberry, kiwi, papaya

• Cereals: Rice, wheat, maize, barley, oats

• Economicals: Coffee, tea, cotton, sugarcane, tobacco

• Medicinals: Ginseng, astragalus, goji berry, echinacea

• Ornamentals: Rose, orchid, chrysanthemum, turfgrass

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NO.4: Cutting-Edge Production Workflow

1. Strain Amplification:

   • P. lilacinum: 28°C, pH 6.8, aerobic fermentation (72h).

   • B. subtilis: 37°C, pH 7.4, fed-batch culture (48h).
     → Centrifugal concentration to 10¹⁰ CFU/mL.

2. Carrier Engineering:
   Sterilized composted straw : bentonite (3:1) adjusted to 35% moisture.

3. Solid-State Fermentation:
   Dual-strain co-inoculation (5% v/w), 28°C, 72h with intermittent aeration.

4. Stabilization:
   Chitosan coating → fluidized-bed drying ≤45°C → packaging.

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NO.5: Operational Safeguards

• Incompatibles: Copper-based fungicides, streptomycin, extreme pH inputs.

• Storage: <30°C, RH<70%, UV-protected packaging.

• Heavy Metals: As<75ppm, Pb<100ppm, Cd≤10ppm, Cr≤150ppm, Hg<5ppm.

• Shelf-Life: 12 months post-manufacture (see batch code).

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Technical FAQ: Microbial Inoculant (Inhibit Root Knot Nematode)

Q1: How does this inoculant suppress nematodes?
A: Purpureocillium lilacinum parasitizes juvenile nematodes and eggs through direct hyphal penetration, while B. subtilis produces nematicidal metabolites (e.g., bacillomycin D), reducing RKN populations by 70–90% within 45 days.

Q2: Can it be tank-mixed with NPK fertilizers?
A: Compatible with most organic/synthetic fertilizers at pH 5.5–8.0. Avoid mixing with anhydrous ammonia or high-salinity (>6 dS/m) solutions – apply sequentially with 48h interval.

Q3: What is the rhizosphere colonization mechanism?
A: Strains utilize root exudates (e.g., malic acid) as chemoattractants. Biofilm formation establishes microcolonies within 72h, creating a protective “microbial shield” against invaders.

Q4: Does it alleviate continuous cropping (soil sickness)?
A: Yes. Degrades phenolic acids (e.g., p-hydroxybenzoic acid) and auto-toxins while rebuilding beneficial microbiome diversity (↑ 30% Actinobacteria).

Q5: Why is brown sugar mandatory for activation?
A: Sugar provides C-source for glycolytic respiration, triggering ATP-dependent spore germination and upregulating adhesion genes (epsD, pelA) critical for root attachment.

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Sustainability Validation:
Third-party trials confirm 22–37% yield increase in nematode-infested fields, 45% reduction in chemical nematicide use, and SOC elevation of 0.8–1.2% after 3 seasons. Deploy this next-generation bio-solution to transform soil resilience and crop profitability.

 

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